ABSTRACT
Objective To investigate the incidence of trisomy 12(+12) and 11 q23 deletion [ del ( 11q23) ] in chronic lymphocyticleukemia (CLL). Methods Fluorescein labeled DNA probe 12 and sequence specific probe ATM for 11q23 were used to perform inter-phase fluorescence in situ hybridization (I-FISH) assays in 30 patients with CLL. The results were compared with that of conventional cyto-genetic (CC) examination. Results With CC examination , only 4 cases (13.3%) were found to have chromosomal abnormalities, whereaswith I-FISH assay ,8 cases (26.7%) were found to have genomic aberrations, including trisomy 12 in 5 cases , deletion of 11q23 in 3 ca-sea. Conclusion I-FISH is a useful method for detection of genomie aberration in CLL, the significance of trisomy 12 and del (11q23) inpredicting the prognosis of B-CLL need to be investigated further.
ABSTRACT
Objective: To analyse the clinical significance of a-L-Fucosidase(AFU) in the diagnosis of primary hepatic carcinoma(PHC).Methods: The activity of serum AFU was measured in 33 patients with PHC,42 patients with cirrhosis,37 patients whit hepatitis,10 patients with secondary liver cancer,37 patients with other malignant tumors and 80 normal persons.The differences of the level of AFU between the groups were compared.Results: The mean serum AFU activity was significantly higher in patients with PHC(41.2?14.1 U/L) than that in normal persons(20.5?7.2U/L),secondary liver cancer(22.2?13.6 U/L),other kinds of malignant tumors(22.0?7.1 U/L),P